Abcam, ab18123, Anti-ATF1 - S.pombe antibody [ATF1 2A9/8]

Size: 50µg
Mouse Monoclonal ATF1 - S.pombe antibody. Suitable for IP, WB and reacts with Schizosaccharomyces pombe samples. Cited in 8 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:ATF1 2A9/8,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Schizosaccharomyces pombe,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
ATF1 in S. pombe is a bZIP transcription factor with strong homology to the mammalian factor ATF1.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATF1 also known as Activating Transcription Factor 1 is a transcription factor in Schizosaccharomyces pombe (S. pombe). ATF1 has a mass of approximately 45 kDa and is expressed in the nucleus. It works as a DNA-binding protein that regulates gene expression by binding to cAMP response elements. ATF1's activity depends on phosphorylation which modulates its function during cellular stress responses.
Biological function summary
ATF1 in S. pombe plays an essential role in stress responses and cell cycle regulation. It acts not as an isolated unit but as part of a transcriptional activation complex with Pcr1. This complex formation allows ATF1 to regulate various genes involved in responses to oxidative stress and osmotic stress ensuring cell survival under adverse conditions. Its activation can lead to the expression of genes needed for cell adaptation and maintenance.
Pathways
ATF1 is heavily involved in the stress-activated protein kinase (SAPK) signaling pathway. In this pathway ATF1 interacts with proteins such as Sty1 which phosphorylates ATF1 promoting its activity. This interaction is an important link in the cascade that enables cellular adaptation to environmental changes. The SAPK pathway illustrates ATF1’s involvement in regulating gene expression responses that ensure proper cellular reaction to stress stimuli.
ATF1's regulation in S. pombe serves as a model for understanding stress response mechanisms relevant in human diseases like cancer. Dysregulation of pathways involving ATF1 can relate to abnormal cellular stress responses and apoptosis potentially leading to carcinogenesis. Other proteins like Sty1 also share connections through these dysregulated pathways and contribute to disease states reflecting their potential roles in stress-induced cellular malfunctions.