Abcam, ab181413, Anti-Osteoblast Marker antibody [ZNS-5]

Size: 100µg
Mouse Monoclonal Osteoblast Marker antibody. Suitable for IHC-P, IHC-Fr and reacts with Zebrafish samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:zns-5,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Zebrafish,
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
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Properties and Storage Information:
Form-Liquid, Purity-undefined SDS-PAGE, Purification notes-Near homogeneity as judged by SDS-PAGE. ab181413 was produced in vitro using hybridomas grown in serum-free medium and then concentrated by chemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: 99% HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Osteoblast markers including well-known markers like alkaline phosphatase (ALP) and osteocalcin play important roles in bone formation. Alkaline phosphatase has a mass around 80-120 kDa and is widely expressed in osteoblasts. Osteocalcin around 49 kDa is secreted by osteoblasts and is an indicator of bone turnover. These markers are key in evaluating bone metabolism and are expressed in bone tissues where bone formation and remodeling occur.
Biological function summary
Osteoblasts are responsible for synthesizing the bone matrix and mineralization. They derive from mesenchymal stem cells and use these markers for identification during the differentiation process. Alkaline phosphatase removes phosphate groups which helps in mineralization while osteocalcin binds calcium and integrates it into the bone matrix. No known complex involves osteoblast markers directly; however they indicate the activity state of osteoblasts within their biological environment.
Pathways
Osteoblast markers are active participants in the Wnt and BMP signaling. The Wnt pathway is important for the regulation of osteoblast differentiation and bone formation where markers like ALP show increased expression. In the BMP pathway bone morphogenetic proteins stimulate osteogenic activity and these markers reflect this stimulatory effect. They interact with proteins such as beta-catenin in the Wnt pathway and various Smad proteins in the BMP pathway.
Osteoblast markers relate to osteoporosis and Paget's disease. In osteoporosis decreased expression of osteoblast markers indicates reduced bone formation. In Paget's disease markers often increase due to abnormal and excessive bone remodeling. The markers frequently associate with proteins such as RANKL which influences bone resorption and OPG which regulates this process. These connections are important for understanding the disruptions in bone homeostasis associated with these diseases.