Abcam, ab181547, Anti-Insulin antibody [EPR17359]

Size: 20µL / 100µL / 1mL
Anti-Insulin antibody [EPR17359] (ab181547) is a rabbit monoclonal antibody detecting Insulin in Western Blot, IHC-P, ICC/IF, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17359,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, mIHC, IHC-FoFr, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The human recommendation is based on the IHC-P results. We do not guarantee WB for human.

Product details:
Product Specifications
Anti-Insulin antibody [EPR17359] (ab181547) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-FoFr, IHC-P, WB, mIHC in human, mouse, rat samples.
Anti-Insulin antibody [EPR17359] (ab181547) specifically detects Insulin (UniProt ID: P01308; Molecular weight: 3kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Insulin antibody [EPR17359] (ab181547) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Insulin antibody [EPR17359] (ab181547) has been cited over 98 times in peer reviewed journals and is trusted by the scientific community.
Anti-Insulin antibody [EPR17359] (ab181547) has 11 independent reviews from customers.
Related Products
Antibody clone EPR17359 is also available pre-conjugated to a variety of labels for your convenience - PE (
ab213192
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary
Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.
Pathways
Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.
Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.