Abcam, ab181991, Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Zonula occludens protein 3/ZO3 antibody. Plasma Membrane marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13414,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Zonula occludens protein 3 (ZO-3) also known as Tight junction protein 3 is a component of tight junction complexes located in epithelial and endothelial cells. ZO-3 exhibits a molecular mass of approximately 130 kDa. Within the cellular environment ZO-3 serves as a scaffold protein anchoring transmembrane proteins to the actin cytoskeleton maintaining tight junction integrity. Expressions of ZO-3 occur in tissues where tight junctions are present including the intestinal epithelium and blood-brain barrier.
Biological function summary
ZO-3 participates with other proteins in maintaining cellular polarity and regulating paracellular permeability. It forms a part of the tight junction complex where it interacts with proteins like ZO-1 and occludin. Through these interactions ZO-3 contributes to the junctional barrier between adjacent cells playing an essential role in controlling the passage of molecules and ions through these cellular layers. ZO-3 also helps in signal transduction pathways linked to cellular proliferation and differentiation.
Pathways
ZO-3 plays a significant role in tight junction signaling and the establishment of epithelial cell adhesion. Within these pathways ZO-3 collaborates with ZO-1 and other junctional proteins forming a network that regulates cellular responses to extracellular stimuli. These associations reinforce the epithelial barrier and help manage processes such as inflammation and tissue repair important for maintaining tissue homeostasis.
Zonula occludens protein 3 has a connection to conditions like inflammatory bowel disease (IBD) and certain cancers. Variations or disruptions in ZO-3 expression can weaken the intestinal barrier having potentially contributing effects on the development of IBD. Furthermore ZO-3 has an association with tumor suppressor proteins like ZO-1 suggesting that its dysfunction might play a role in the progression of cancerous growths by altering barrier properties and influencing cell proliferation.