Abcam, ab182422, Anti-CD163 antibody [EPR19518]

Size: 20µL / 100µL / 1mL
Anti-CD163 antibody [EPR19518] (ab182422) is a rabbit monoclonal antibody detecting CD163 in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, mIHC . Suitable for Human, Mouse, Rat . - Clone EPR19518 is the most cited clone to CD163 - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 390 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19518,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, mIHC, IHC-Fr, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD163 antibody [EPR19518] (ab182422) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, IHC-Fr IHC-P, WB and mIHC.
Anti-CD163 antibody [EPR19518] (ab182422) was first used in a scientific publication in 2014 and has been cited over 399 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD163 antibody [EPR19518] (ab182422) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD163 antibody [EPR19518] (ab182422) has 17 independent reviews from customers.
Anti-CD163 antibody [EPR19518] (ab182422) specifically detects CD163 (UniProt ID: Q2VLH6; Molecular weight: 118kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR19518 -
ab213612
Antibody clone EPR19518 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
555, Alexa Fluor
594, Alexa Fluor
488, Alexa Fluor
647, Biotin, PE (
ab281746
ab282114
ab313665
ab313666
ab314941
ab314947
Recombinant antibody to CD163 is involved in conditions like rheumatoid arthritis and atherosclerosis, where it helps regulate inflammation. Top cited clone on the market
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary
CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.
Pathways
CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.
CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.