Abcam, ab182981, Anti-CD31 antibody [EPR17259]

Size: 20µL / 100µL / 1mL
Anti-CD31 antibody [EPR17259] (ab182981) is a rabbit monoclonal antibody detecting CD31 in IHC-P, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 220 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17259,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:mIHC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD31 antibody [EPR17259] (ab182981) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P and mIHC.
Anti-CD31 antibody [EPR17259] (ab182981) was first used in a scientific publication in 2018 and has been cited over 224 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD31 antibody [EPR17259] (ab182981) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD31 antibody [EPR17259] (ab182981) has 19 independent reviews from customers.
Anti-CD31 antibody [EPR17259] (ab182981) specifically detects CD31 (UniProt ID: Q08481; Molecular weight: 80kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR17259 -
ab225883
Antibody clone EPR17259 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, Alexa Fluor
488, Alexa Fluor
ab305210
ab305267
ab312892
CD31 (PECAM-1) is a protein expressed on endothelial and immune cells, playing a crucial role in tumor angiogenesis and immune cell trafficking. In immuno-oncology, it influences the tumor microenvironment and modulates the immune response to cancer.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD31 also known as Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) is a protein with a mass of approximately 130 kDa. This protein is an important component of the cell surface and is expressed mainly in endothelial cells and certain leukocyte populations. It plays a role in cell-cell adhesion and is recognized as an important marker in immunohistochemistry (IHC) for identifying endothelial cells. CD31 is valuable for researchers focusing on vascular biology and immunology serving as a beacon for cells involved in forming blood vessels or those located within the vascular system.
Biological function summary
CD31 is important for the transendothelial migration of leukocytes. It forms complex interactions with itself (homophilic binding) and other partners to mediate processes necessary for immune response and tissue integrity. This protein supports the development and maintenance of endothelial cell junctions making it fundamental in maintaining vascular permeability. Additionally CD31 plays a role in transmitting signals that influence the survival of leukocytes and endothelial cells. Its expression and function help regulate the balance between cell life and death impacting immune function and tissue repair.
Pathways
CD31 participates actively in both immune and angiogenesis pathways. It associates with proteins like SHP-2 and SHP-1 which are involved in downstream signaling cascades critical for cell signaling and immune cell activation. Within the angiogenesis pathway CD31 interacts with other adhesion molecules to promote new blood vessel formation under physiological and pathological conditions. It also collaborates with integrins influencing cellular responses to external stimuli and contributing to vascular remodeling and repair.
CD31 is significantly associated with inflammatory conditions and cardiovascular diseases. For instance its dysfunction or altered expression can contribute to atherosclerosis a disease characterized by the hardening of arteries due to plaque buildup. Furthermore CD31 deficiencies have links with autoimmune disorders where it might affect leukocyte migration and contribute to tissue damage. The protein associates with ICAM-1 during inflammatory processes illustrating its complex involvement in disease progression and potential as a therapeutic target.