Abcam, ab183311, Anti-C4d antibody [SP91] - C-terminal

Size: 20µL / 100µL / 500µL / 1mL
Anti-C4d antibody [SP91] - C-terminal (ab183311) is a rabbit monoclonal antibody detecting C4d in IHC-P . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP91,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-C4d antibody [SP91] - C-terminal (ab183311) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
Collaboration
Anti-C4d [SP91] - C-terminal (ab183311) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Other related products
We have a range of other formats of antibody clone [SP91] also available for your convenience: ab183311, Carrier free -
ab236231
, Alexa Fluor® 488 -
ab320605
, Alexa Fluor® 647 -
ab320606
, Alexa Fluor® 555 -
ab320607
, Alexa Fluor® 594 -
ab320608
, PE -
ab320609
, APC -
ab320610
, Alexa Fluor® 750 -
ab321266
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C4d also known as the C4d complement is a degradation product of the complement component C4. It has a molecular weight of approximately 45 kDa. This protein is commonly expressed on the surfaces of endothelial cells in various tissues where it plays a role in the immune response. The presence of C4d on tissues is often detected using techniques like immunohistochemistry (IHC) also referred to as C4d IHC or C4d immunostain providing valuable insights into complement activation.
Biological function summary
The C4d protein acts as a marker for complement activation and is part of the larger complement cascade. C4d is primarily associated with the classical pathway and serves as an indicator of complement-mediated cell injury. The degradation of C4 into C4d is usually seen in immune responses where antibodies recognize antigens triggering sequence activation in the complement pathway. C4d's role as a marker facilitates the differentiation between antibody-mediated injury and other types of tissue damage.
Pathways
This complement protein gets involved in the classical pathway of the complement system which plays a critical role in innate and adaptive immunity. It is related to other proteins like C3 and C1q which are additional components within this pathway. These interactions are significant for the clearance of pathogens and also assist in enhancing the humoral immune response by promoting the deposition of complement on pathogenic surfaces.
C4d presence becomes particularly significant in conditions like transplant rejection and systemic lupus erythematosus (SLE). In transplant rejection its deposition on tissue samples might indicate antibody-mediated rejection where C4d binds to the tissue signaling an immune response against the transplanted organ. Additionally in SLE C4d's relation to other proteins such as C3 and C1q confirms its involvement in autoimmune responses where the immune system mistakenly targets the body's own tissues leading to chronic inflammation and damage.