Abcam, ab183360, Human FAS ELISA Kit

Size: 1 x 96Tests
Human FAS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FAS with a sensitivity of 26 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 26 pg/mL,
Range:46.88 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
As of October 15, 2019, Human FAS ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human FAS ELISA kit (ab183360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAS protein in human cell culture supernatant, serum, and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human FAS with 2 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
FAS is a plasma membrane receptor for FASLG ligand (TNFSF6/FASLG). Upon ligand binding, the FASLG Receptor is activated and the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) activation mediating apoptosis. FASLG-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 of the FASLG Receptor block apoptosis as they sequester the Fas ligand.
This kit uses a recombinant protein fragment corresponding to the extracellular portion (amino acid 17-172) of the full length Human Fas Receptor protein as standard sample.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C