Abcam, ab183516, Anti-ATG4C antibody [EPR15001]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ATG4C antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15001,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATG4C also known as autophagin-3 is a cysteine protease involved in autophagy an important cellular degradation pathway. With a molecular mass of approximately 52 kDa the target plays a significant role in cytosolic processing. Primarily ATG4C acts in various tissues including the brain liver and muscle where it participates in the processing of essential autophagy-related proteins. The protein provides an enzymatic function necessary for the maturation and recycling of autophagosomes structures required for autophagy.
Biological function summary
ATG4C cleaves the ATG8 family proteins such as LC3 at a conserved glycine residue facilitating their conjugation to phosphatidylethanolamine. This step is critical in the elongation of the isolation membrane during autophagosome formation. ATG4C is not typically found as part of a large protein complex but its interaction with ATG8 family members is an important regulatory point. Its function in recycling ATG8-family proteins helps maintain cellular homeostasis and responds to stress conditions like nutrient deprivation.
Pathways
ATG4C plays a central role in the autophagy pathway essential for cell survival differentiation and tissue remodeling. The protein closely interacts with components of the autophagy machinery such as ATG5 and the ULK1 complex coordinating the effective sequestration of cytoplasmic constituents. Its activity impacts the broader cellular pathways involving mTOR and AMPK both of which regulate cellular energy status and respond to metabolic signals positioning ATG4C as a vital component in these pathways.
Alterations in ATG4C function have been linked to cancer and neurodegenerative disorders. In cancer dysregulated autophagy affects tumor growth and therapy resistance. ATG4C is related to Beclin 1 which regulates autophagy initiation in tumors. In neurodegeneration impaired autophagy exacerbates the accumulation of protein aggregates. Misfunction of ATG4C can influence these processes showing a direct connection to p62/SQSTM1 a protein involved in defective degradation that leads to neurodegenerative pathology.