Abcam, ab183734, Anti-GFP antibody [EPR14104]

Size: 20µL / 100µL / 1mL
Anti-GFP antibody [EPR14104] (ab183734) is a rabbit monoclonal antibody detecting GFP in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . - Biophysical QC for unrivalled batch-batch consistency - Over 80 publications See more GFP antibodies and assay kits
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14104,
Isotype:IgG,
Carrier free:No,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:On the basis of low sequence homology, ab183734 is predicted to show no or limited cross-reactivity to RFP and BFP.For the best results in IHC, please use ab183734 on Formalin/PFA-fixed paraffin-embedded sections with heat mediated antigen retrieval.

Product details:
Anti-GFP antibody [EPR14104] (ab183734) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Abcam's high quality manufacturing and validation processes ensure Anti-GFP antibody [EPR14104] (ab183734) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GFP antibody [EPR14104] (ab183734) has 24 independent reviews from customers.
GFP antibodies are often used as loading controls in Western Blot. Anti-GFP antibody [EPR14104] has been verified in Western Blot samples and detects a band at 27kDa Molecular weight.
GFP antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-GFP antibody [EPR14104] (ab183734) has been validated in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-GFP antibody [EPR14104] (ab183734) specifically detects GFP (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR14104 -
ab220802
Antibody clone EPR14104 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, APC, PE (
ab225314
ab283597
ab303588
Green Fluorescent Protein (GFP), originally derived from the jellyfish Aequorea victoria, emits a bright green fluorescence under ultraviolet or blue light. This unique property makes GFP an invaluable tool in molecular and cell biology research. Widely used to tag proteins, GFP allows scientists to visualize and track protein expression, localization and interactions within living cells. Key applications of GFP include fluorescence microscopy (ICC, IHC, IF), flow cytometry (FC) and western blotting (WB).
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle