Abcam, ab184601, Anti-EGFP antibody [F56-6A1.2.3]

Size: 100µg
Anti-EGFP antibody [F56-6A1.2.3] (ab184601) is a mouse monoclonal antibody detecting EGFP in Western Blot, ICC/IF . - Over 40 publications See more GFP antibodies and assay kits
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:F56-6A1.2.3,
Isotype:IgG2b,
Carrier free:No,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Anti-EGFP antibody [F56-6A1.2.3] (ab184601) is a House Mouse Monoclonal antibody and is validated for use in ICC/IF, WB in human, mouse samples.
Anti-EGFP antibody [F56-6A1.2.3] (ab184601) has been cited over 40 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-EGFP antibody [F56-6A1.2.3] (ab184601) has high sensitivity and specificity.
Anti-EGFP antibody [F56-6A1.2.3] (ab184601) specifically detects EGFP (UniProt ID: ; Molecular weight: kDa) and is sold in 100 ug selling sizes.
Enhanced Green Fluorescent Protein (EGFP) is a modified version of the original green fluorescent protein (GFP) derived from the jellyfish Aequorea victoria. EGFP is widely used in molecular and cellular biology due to its bright fluorescence and stability. It serves as a powerful tool for visualizing and tracking proteins, cells, and cellular processes in real-time. EGFP is employed in various research applications, including gene expression studies, protein localization, and cell lineage tracing.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-in vitro produced, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EGFP or Enhanced Green Fluorescent Protein is a widely used marker in molecular biology. Known also as GFP EGFP its expression occurs in various organisms and facilitates easy visualization of cellular and molecular processes. The molecular weight of EGFP is approximately 27 kDa contributing to its manageable size for integration into other proteins. Generally scientists observe EGFP in tissues and cell types where it is used as a fusion tag to monitor protein localization and dynamics.
Biological function summary
EGFP functions without disrupting the normal cellular environment where it fluoresces green under ultraviolet or blue light. The protein does not form a complex with other molecules allowing it to remain stable and functional in diverse conditions. Scientists often utilize the EGFP sequence to create fusion proteins aiding in the study of protein-protein interactions trafficking and other cellular processes. This versatility makes EGFP a valuable tool in biological experiments.
Pathways
Scientists exploit EGFP as a marker within research on cellular signaling and gene expression pathways. It is particularly useful within the analysis of the Ras signaling pathway and the NF-kB pathway where it can help track the movement and interaction of key proteins such as Ras and IκB. The fluorescent characteristics of EGFP allow real-time observation of these pathways enhancing the understanding of molecular processes in live cells.
EGFP serves as a critical tool in cancer research and neurodegenerative diseases. Through its fusion with proteins like p53 a tumor suppressor researchers can track gene expression and protein stability in cancerous cells. Similarly by merging EGFP with proteins such as tau linked to Alzheimer's disease scientists investigate protein aggregation and neuron behavior in neurodegenerative conditions. Therefore EGFP remains integral to disease research offering insights into the underlying molecular mechanisms.