Abcam, ab185699, Anti-EPPIN antibody

Size: 100µL
Rabbit Polyclonal EPPIN antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPPIN.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human EPPIN.O95925

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EPPIN short for Epididymal protease inhibitor is a protein that plays an important structural and mechanical role in sperm functionality. With a molecular mass of approximately 19 kDa EPPIN is mainly expressed in the male reproductive system particularly in the epididymis and on the surface of spermatozoa. This protein is recognized by several names including SPINLW1 which stands for Sperm protein with a Kunitz-type inhibitor domain. EPPIN interacts with the surface of sperm to regulate sperm motility and maturation.
Biological function summary
EPPIN influences sperm function by acting as part of a multi-protein complex essential for sperm motility and stability. It is known to bind with Semenogelin (SEMG) a major protein in semen impacting its hydrolytic and inhibitory action. Through this interaction EPPIN contributes to the liquefaction of semen and the regulation of sperm forward motility. It modulates calcium signaling in spermatozoa which is critical for the regulation of sperm activity post-ejaculation.
Pathways
EPPIN has been implicated in pathways associated with reproductive processes. It plays a role in the regulation of the calcium signaling pathway which involves other proteins like CatSper which are essential for sperm capacitation and hyperactivation. EPPIN through its interaction with SEMG indirectly influences the proteolytic pathways that govern seminal fluid conversion from gel to liquid state necessary for the sperm to become fully motile.
EPPIN has connections with male infertility and potential as a target for male contraception. Its interaction with SEMG has been studied in relation to infertility cases where sperm motility is compromised. Furthermore altered levels or functions of EPPIN could link to abnormal semen viscosity contributing to difficulties in natural conception. Researchers are exploring EPPIN as a target for non-hormonal male contraceptives leveraging its pivotal role in sperm functionality without affecting hormone levels.