Abcam, ab185917, Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry)

Size: 50Test / 200Test
Fix & Perm / Cell fixation and permeabilization kit is designed to improve the staining of intracellular markers for flow cytometry by fixing cells and permeabilizing cell membranes, enabling access to cytoplasmic, mitochondrial, nuclear, and other organelle markers.
Product details:
Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.
The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.
The kit protocol is very simple:
- incubate cells with Fixation Medium for 15 min
- add PBS and spin down cells
- add Permeabilization Medium and antibody to cell pellet
- incubate for 15 min
- wash cells with PBS and analyze
The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).

Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient