Abcam, ab185976, Anti-TAP tag antibody [22F12-1E3] - C-terminal

Size: 50µg
Mouse Monoclonal TAP tag antibody. C-terminal. Suitable for WB and reacts with Tag samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:22F12-1E3,
Isotype:IgG1,
Carrier free:No,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
TAP adds about 20 kDa to the size of the protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TAP tag also known as the Tandem Affinity Purification tag is a biotechnological tool used for studying protein interactions and functions. It is not a natural protein but rather an engineered tag that researchers attach to target proteins for purification and analysis purposes. The TAP tag comprises two protein domains typically Protein A and calmodulin-binding peptide connected by a TEV protease cleavage site and has a total mass of approximately 21 kDa. Researchers frequently express TAP-tagged proteins in yeast cells or mammalian systems to facilitate the study of complex protein networks in these models.
Biological function summary
TAP tags allow for the isolation and analysis of protein complexes from cells. This occurs through a multi-step purification process leveraging the tag's affinity for specific binding partners. Using TAP tags enhances the identification and study of interacting proteins and complexes improving our understanding of biological processes. The TAP tag system is often combined with mass spectrometry to identify additional complex members enhancing the resolution of protein interaction networks.
Pathways
TAP tags enable the investigation of proteins involved in key pathways by isolating their associated complexes. Notable pathways where TAP tags have facilitated research include signal transduction and cell cycle regulation. Within these pathways TAP tags help identify interactions with proteins like kinases and phosphatases providing insights into pathway dynamics and control mechanisms. The use of TAP tagging in pathway analysis can also reveal cross-talk between pathways by uncovering shared or sequentially interacting proteins.
TAP tags have been instrumental in revealing interactions relevant to cancer and neurodegenerative diseases. Researchers have used TAP tags to study molecules involved in tumor progression and metastasis shedding light on proteins like p53 and its network of interactions. Similarly in neurodegenerative conditions such as Alzheimer's disease TAP tags facilitate the identification of protein complexes that include beta-amyloid precursor protein (APP) and associated interactors offering insights into pathogenic mechanisms. These studies can help identify new biomarkers or therapeutic targets by elucidating the complex protein interactions modulating disease processes.