Abcam, ab18598, Biotin Anti-Protein A antibody

Size: 500µg
Anti-Protein A antibody - Biotin conjugated (ab18598) is a chicken polyclonal antibody detecting Protein A in Western Blot, ELISA . Suitable for Staphylococcus aureus . - Trusted since 2005
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Conjugation:Biotin,
Carrier free:No,
Reacts with:Staphylococcus aureus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus NCTC 8325 spa.P02976

Product details:
What is this antibody validated in?
Biotin Anti-Protein A antibody (ab18598) is a chicken polyclonal antibody and is validated for use in Western Blot (WB), ELISA in Staphylococcus aureus samples.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin., Storage buffer-pH: 7.2Preservative: 0.1% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein A also known as Staphylococcus aureus Protein A is a bacterial protein with a molecular weight of approximately 42 kDa. It is found in the cell wall of the bacterium Staphylococcus aureus. This protein binds strongly to immunoglobulins especially the Fc region of IgG making it a useful tool in biochemical research. This binding property is used to isolate antibodies and also in various immunoassays such as Protein A ELISA kits.
Biological function summary
Protein A from Staphylococcus aureus plays a role in the evasion of host immune responses. It helps the bacterium avoid detection by immune cells aiding in its pathogenicity. Protein A does not form part of any large complex but interacts directly with IgG molecules. This ability to bind antibodies is leveraged in the laboratory to purify antibody-antigen complexes which can be critical for research and diagnostic applications.
Pathways
Its role is more mechanical than part of any classic biochemical pathways but it contributes to evasion of the host immune pathway. It has connections with immune signaling proteins such as the Fc receptors found on immune cells. The interception and binding of antibodies by Protein A can modulate the normal immune signaling pathways thereby impacting immune surveillance and response.
Protein A's interaction with the immune system links it to conditions involving Staphylococcus aureus infections such as pneumonia and skin infections. Its ability to interfere with antibody function contributes to the virulence of Staphylococcus aureus and complicates the immune response. Its connection to Fc receptor proteins illustrates its potential role in evading immune reactions during the infectious disease process presenting challenges in treatment and diagnosis.