Abcam, ab186027, Cellular ROS Assay Kit (Red)

Size: 200Test
Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in cell cultures. - Add ROS sensor reagent to cell cultures, treat cells to induce ROS production, and readout on a fluorometric (Ex/Em 520/605 nm) plate reader. - Cited in >50 publications
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based (quantitative),
Assay time:60m,
Assay Platform:Microplate reader, Fluorescence microscope

Product details:
Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells. The red dye used in the ROS assay protocol is cell-permeable and generates red fluorescence when it reacts with ROS. The kit is an optimized "mix and read" assay format that is compatible with HTS liquid handling instruments.
ab186027 provides an ultrasensitive fluorometric one-step ROS assay. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 520/605 nm and can be used to either quantify ROS levels in cells or to screen ROS inhibitors.
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.
Related ROS assay kits
To measure reactive oxygen species within cells, we recommend DCFDA assay kit
ab113851
. Alternative ROS assays are available in orange (
ab186028
), red (ab186027), and deep red (
ab186029
). DCF ROS/RNS Assay Kit
ab238535
is used to measure ROS in biofluids, culture supernatants and cell lysates.
For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use
ab139476
; to assay ROS, superoxides, and reactive nitrogen species use
ab139473
; to assay superoxides use
ab219943
Other notes
Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Red Fluorescence).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C