Abcam, ab186035, SMAD1 (pS463/S465 + Total SMAD1) ELISA Kit

Size: 1 x 96Tests
SMAD1 (pS463/S465 + Total SMAD1) ELISA Kit is a single-wash 90-min Simplestep used to quantify SMAD1 (pS463/S465 + Total SMAD1) with a sensitivity of 20 µg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Tissue Homogenate, Cell Lysate,
Reacts with:Mouse, Human,
Assay type:Semi-quantitative,
Sensitivity:= 20 µg/mL,
Range:10 - 1000 µg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
SMAD1 (pS463/S465) and SMAD1 (Total)
in vitro
SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of SMAD (pS463/S465) and Total SMAD1 protein in human and mouse cells.
The SimpleStep ELISA™ employs a labeled capture and detector antibody which immunocaptures the sample analyte in solution. This entire complex (capture antibody/protein/detector antibody) is in turn immobilized in the well by immunoaffinity via the anti-tag antibody. Samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material; the TMB substrate is then added. The reaction is stopped by addition of Stop Solution which stops the color development and completes any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
ASSAY SPECIFICITY
The SMAD1 (pS463/S465) assay detects endogenous levels of SMAD1 (GenBank Accession NP_001003688) in cellular lysates, only when phosphorylated at Ser463/465. Based on sequence similarity, cross reaction to SMAD5 and SMAD8 may occur.
The SMAD1 (Total) assay detects endogenous levels of SMAD1 (GenBank Accession NP_001003688) in cellular lysates. Total SMAD1 assay kits detect SMAD1, irrespective of phosphorylation status. Based on sequence similarity, cross reaction to SMAD5 and SMAD8 may occur.
SPECIES REACTIVITY
This kit detects SMAD1 (pS463/S465) and SMAD1 (Total) in Human and mouse cell culture extracts. Detection in rat samples is also expected. Other species should be tested on a case-by-case basis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Smad1 also known as Mothers Against Decapentaplegic Homolog 1 is a protein that functions as an intracellular messenger. Its molecular weight is approximately 60 kDa. Smad1 is expressed in various tissues with higher levels found in bone and cartilage tissues. Mechanically Smad1 transmits signals from the cell membrane to the nucleus. It gets activated upon phosphorylation by receptor-regulated Smad kinases specifically. Once phosphorylated Smad1 forms a complex with Smad4 which enables its movement into the nucleus to regulate gene expression.
Biological function summary
Smad1 plays a central role in bone morphogenetic protein (BMP) signaling pathways. It serves as part of a multiprotein complex allowing it to exert its functions effectively. This protein impacts cellular processes such as proliferation differentiation and apoptosis. It is very important in the development of tissues that require BMP signals. Through its ability to regulate gene expression depending on external BMP signals Smad1 contributes to the modulation of embryogenesis and tissue homeostasis.
Pathways
Smad1 participates in the transforming growth factor-beta (TGF-beta) signaling pathway and the BMP signaling pathway. The BMP pathway significantly influences bone formation and maintenance. Within this network Smad1 closely interacts with other proteins like BMP receptors and Smad4. In the context of TGF-beta signaling Smad1 aligns functionally alongside Smad2 and Smad3 although typically it is more BMP-specific. The interconnectedness with these pathways highlights Smad1's importance in cellular responses to growth factors.
Smad1 has connections to various conditions notably cancer and fibrodysplasia ossificans progressiva (FOP). Alterations or mutations in Smad1 signaling can lead to uncontrolled cell growth and cancer development due to the disruption of normal cell cycle control. In FOP aberrant signaling involving Smad1 leads to the formation of bone in soft tissues. This disorder also involves protein interactions with ACVR1 a type I BMP receptor which drives the pathological ossification process.