Abcam, ab186869, Anti-Constitutive androstane receptor antibody

Size: 100µL
Rabbit Polyclonal Constitutive androstane receptor antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR1I3 aa 100 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NR1I3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q14994

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Constitutive Androstane Receptor (CAR) also known as NR1I3 (nuclear receptor subfamily 1 group I member 3) is a nuclear receptor and transcription factor with a molecular weight of approximately 40 kDa. Structurally it plays an important role in regulating the expression of genes involved in drug metabolism and clearance. CAR is highly expressed in the liver with lower levels in the intestine. It acts as a ligand-activated transcription factor undergoing conformational change upon ligand binding that facilitates interaction with other transcriptional regulators.
Biological function summary
CAR impacts the detoxification processes of xenobiotics and endogenous compounds. It does not function alone; instead it forms a heterodimer with Retinoid X Receptor (RXR) to bind DNA response elements. This interaction leads to the induction or repression of various genes. The dimerization with RXR is important for the activation of target genes involved in phase I and phase II drug metabolism influencing enzymes such as cytochrome P450s.
Pathways
CAR's role is significant in the xenobiotic and bilirubin clearance pathways. It regulates the transcription of enzymes in the cytochrome P450 family particularly CYP2B6 and CYP3A4. This protein has connections with other nuclear receptors like Pregnane X Receptor (PXR) which shares partial overlap in target genes assisting in the detoxification pathway. Both receptors contribute to a comprehensive regulation of hepatic drug metabolism.
CAR's function affects conditions like liver diseases and metabolic syndromes. Dysregulation of CAR activity links to liver carcinogenesis through its role in hepatic xenobiotic metabolism. Additionally alterations in CAR activity affect lipid metabolism potentially leading to metabolic disorders. Interacting proteins such as PXR also partake in these processes providing further insight into disease mechanisms influenced by this receptor.