Abcam, ab187142, Anti-APPBP1 antibody [EPR15732]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal APPBP1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15732,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:Flow Cyt (Intra), WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein 'APPBP1' also known as APP-binding protein 1 functions as part of cellular processes through its role in protein modification. APPBP1 forms a dimeric complex with Uba3 together activating NEDD8 which is a small ubiquitin-like modifier. This protein has a molecular weight of approximately 58 kDa and is widely expressed across various tissues including the brain and liver. Its comprehensive roles make it essential for normal cellular operations.
Biological function summary
APPBP1 interacts within a complex known as the NEDD8-activating enzyme. This complex is essential in the neddylation pathway influencing protein degradation through the ubiquitin-proteasome system. By conjugating NEDD8 to target proteins APPBP1 controls many processes such as cell cycle regulation signal transduction and transcription. It is of special interest in research involving cellular signaling because neddylation modifies the activity and stability of many substrate proteins involved in these critical cellular functions.
Pathways
The involvement of APPBP1 in ubiquitin-like protein pathways highlights its influence on cellular homeostasis. The protein is associated with the neddylation and ubiquitination pathways influencing other members such as cullin proteins involved in cell cycle control and other cellular processes. APPBP1-related interactions play a significant role in ensuring the timely degradation of specific proteins maintaining balance within the cell's regulatory mechanisms.
Abnormalities in APPBP1 activity have been linked to neurodegenerative diseases such as Alzheimer's disease and cancer. In Alzheimer's disease APPBP1 is related to the APP protein and altered neddylation might contribute to disease pathology through dysfunctional protein degradation pathways. In cancer disrupted neddylation can result in uncontrolled cell proliferation due to improper regulation of cell cycle pathways. Understanding the function of APPBP1 could provide insights into developing therapeutic strategies for these conditions.