Abcam, ab187511, Anti-SLC4A4/NBC antibody

Size: 100µL
Rabbit Polyclonal SLC4A4/NBC antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC4A4 aa 1-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SLC4A4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q9Y6R1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.05% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC4A4 also known as NBC (electrogenic Na+/bicarbonate cotransporter) is a protein that functions in the active transport of bicarbonate ions across cell membranes coupled with sodium ions. This cotransporter plays an essential role in maintaining the acid-base balance and cellular pH homeostasis. The SLC4A4 protein has a molecular mass of approximately 121 kDa. It expresses in various tissues with high levels found in the kidney and pancreas. Its activity is critical for efficient transport processes in epithelial cells contributing to the overall ion balance within these organs.
Biological function summary
The activity of SLC4A4/NBC influences several physiological processes. This protein helps regulate bicarbonate reabsorption in renal proximal tubules which is fundamental for the body's buffering capacity. SLC4A4 can operate within complex systems involving other transport mechanisms to efficiently maintain proper ion concentrations and pH balance. It associates with proteins like carbonic anhydrase which facilitates the rapid interconversion of carbon dioxide and bicarbonate enhancing the efficiency of its transport function.
Pathways
SLC4A4/NBC integrates into several key biological pathways that maintain bicarbonate and acid-base equilibrium. One such pathway is the renal tubular bicarbonate reclamation where it collaborates closely with proteins such as NHE3 (sodium hydrogen exchanger 3) to ensure sodium and bicarbonate absorption. Another pathway is the pancreatic bicarbonate secretion where its role complements secretor proteins to assimilate bicarbonates essential for neutralizing gastric acids.
SLC4A4/NBC correlates with significant clinical conditions affecting bicarbonate transport. Mutations or dysfunction in this transporter relate to proximal renal tubular acidosis a disorder characterized by impaired acid-base regulation in kidneys. Additionally it associates with migraine disorders where alterations in pH and ion flow might play a role. Through these conditions SLC4A4 may connect with other bicarbonate transporters such as SLC4A7 to provide insights into pathophysiological mechanisms involving ionic imbalances.