Abcam, ab187679, Anti-Rag D antibody - N-terminal

Size: 100µg
Rabbit Polyclonal Rag D antibody. N-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RRAGD aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RRAGD aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9NQL2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rag D also known by its alternate names RAGA or GTRAG4 is a 40 kDa protein that serves as a regulator of intracellular nutrient signaling. Rag D is expressed in various cell types across numerous tissues. Mechanically it functions as a GTPase cycling between inactive GDP-bound and active GTP-bound states. This cycling is essential for its role in cellular metabolism and growth regulation.
Biological function summary
Rag D participates in the mTORC1 complex a protein complex that senses nutrient availability. It acts as a molecular switch signaling cells to grow or stop growth based on amino acid presence. Rag D facilitates mTORC1 activation by recruiting it to the lysosomal surface where amino acids act as a trigger. This function allows Rag D to play a significant role in cell growth division and metabolism.
Pathways
Rag D integrates into the amino acid sensing pathway directly affecting the mTOR signaling pathway important for cellular growth and homeostasis. Rag D interacts with other Rag proteins such as Rag A and Rag C to form heterodimers that regulate mTORC1 activity. This interaction ensures Rag D can sense and respond to intracellular nutrient levels contributing to homeostatic balance.
Abnormal Rag D function relates to cancer and metabolic conditions like diabetes. Dysregulated mTORC1 signaling where Rag D is an important component has been implicated in tumorigenesis and insulin resistance. Rag D interactions with proteins like TSC2 which modulate mTORC1 activity highlight its relevance in disease states where nutrient sensing is disrupted.