Abcam, ab188027, Anti-ATRX antibody [CL0537]

Size: 100µL
Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody detecting ATRX in Western Blot, IHC-P . Suitable for Human . - KO validated for confirmed specificity
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL0537,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATRX aa 2250-2450. The exact immunogen used to generate this antibody is proprietary information.P46100

Product details:
What is this antibody validated in?
Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of ATRX?
Anti-ATRX [CL0537] (ab188027) specifically detects a band for ATRX (UniProt: P46100) at a molecular weight of 282kDa.
Specificity confirmed
The specificity of Anti-ATRX antibody [CL0537] (ab188027) has been confirmed by Western blot testing in ATRX Knockout HAP1 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ATRX protein also recognized as alpha thalassemia/mental retardation syndrome X-linked (ATR-X) is a chromatin remodeler with a molecular weight of approximately 280 kDa. Expressed predominantly in the brain and thymus ATRX plays an important role in regulating gene expression by modulating chromatin structure. Its expression in neural tissues suggests its importance in brain development where it aids in transcriptional regulation and maintenance of genomic stability.
Biological function summary
ATRX functions by forming part of the ATRX-DAXX complex. This complex plays a vital role in the deposition of the histone variant H3.3 into heterochromatin regions affecting the structure and function of these genomic segments. ATRX through the ATRX-DAXX complex also maintains telomere integrity by preventing uncontrolled telomere elongation. As such ATRX is important in cellular processes like DNA replication repair and mitotic progression emphasizing its role in maintaining genetic fidelity across cell divisions.
Pathways
ATRX is an important component in chromatin remodeling and telomere maintenance pathways. It interacts closely with the DAXX protein within these pathways affecting the histone exchange mechanisms. ATRX also engages with other proteins such as p53 integrating its function into the DNA damage repair pathway. Through these pathways ATRX ensures proper chromatin dynamics and participates in mechanisms guarding genomic stability.
ATRX mutations have significant implications for conditions such as alpha thalassemia/mental retardation syndrome and certain cancers. The protein's altered functions can lead to alpha thalassemia due to improper blood cell gene expression and mental retardation from impaired brain development. In cancers mutations in ATRX often disrupt telomere maintenance contributing to oncogenesis. The relationship between ATRX and telomerase highlights the complex network through which ATRX mutations can influence telomere dynamics in cancer progression.