Abcam, ab188224, Anti-p21 antibody [EPR18021]

Size: 20µL / 100µL / 1mL
Anti-p21 antibody [EPR18021] (ab188224) is a rabbit monoclonal antibody detecting p21 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 160 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18021,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IP, IHC-P, Flow Cyt (Intra), WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expression levels of the target protein vary between different tissue/cell lines and in some cases induction may be required before a signal is observed.This antibody is not recommended for use in WB with tissue samples.

Product details:
Anti-p21 antibody [EPR18021] (ab188224) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Anti-p21 antibody [EPR18021] (ab188224) was first used in a scientific publication in 2017 and has been cited over 163 times in peer reviewed journals. It's performance in Western Blot in mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-p21 antibody [EPR18021] (ab188224) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-p21 antibody [EPR18021] (ab188224) has 11 independent reviews from customers.
Anti-p21 antibody [EPR18021] (ab188224) specifically detects p21 (UniProt ID: P39689; Molecular weight: 18kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR18021 -
ab232512
Antibody clone EPR18021 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, APC, PE (
ab237264
ab237265
ab313956
ab314287
p21 is a cyclin-dependent kinase inhibitor that regulates cell cycle progression, promoting cell cycle arrest in response to DNA damage. It plays a dual role in oncology by both suppressing tumor growth through cell cycle inhibition and contributing to cancer progression when localized in the cytoplasm.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.
Biological function summary
P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.
Pathways
P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.
P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.