Abcam, ab188907, Anti-GPCR GPR65/TDAG8 antibody

Size: 50µg
Rabbit Polyclonal GPCR GPR65/TDAG8 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GPR65.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GPR65. The exact immunogen used to generate this antibody is proprietary information.Q8IYL9,
Specificity:BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPR65 also known as TDAG8 is a G-protein-coupled receptor with an approximate mass of 37.8 kDa. This receptor is involved in sensing extracellular pH changes. It predominates in expression within immune cells including peripheral blood mononuclear cells (PBMCs) and is abundant in tissues like the lung and spleen. The receptor primarily localizes to the plasma membrane where it plays a role in cell signaling pathways by coupling with G-proteins.
Biological function summary
GPR65 contributes to the regulation of immune response and is implicated in the physiological process of proton-sensing. It is a part of signaling complexes that mediate the cellular response to acidic environments influencing processes like inflammation. The receptor modulates the cAMP pathway responding to extracellular acidity and impacting downstream effects on immune cell behavior such as cytokine production and cell migration.
Pathways
GPR65 is associated with the cyclic AMP (cAMP) signaling pathway and the acid-sensing pathway. It interacts with other proteins such as adenylyl cyclase to modulate cAMP levels influencing immune responses and cellular adaptations to acidic environments. GPR65 connects to pathways involving inflammatory responses and shows synergies with pathways regulated by proteins like GPR4 and OGR1 which also function as proton-sensing receptors.
GPR65 is linked to inflammatory conditions and psychiatric disorders. Studies suggest that dysregulation of GPR65 activity can contribute to inflammatory bowel diseases through abnormal immune responses. The receptor may also play a role in mood disorders where its function and expression levels correlate with altered neurochemical environments. It interacts with immune-modulatory proteins including potentially TNF-alpha affecting inflammation and influencing pathological processes.