Abcam, ab189217, Anti-alpha + beta Synuclein antibody [EP1646Y] - BSA and Azide free

Size: 100µg / 1mg
Anti-alpha + beta Synuclein antibody [EP1646Y] - BSA and Azide free (ab189217) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for Western Blot, Flow Cytometry, ICC/IF in Human, Mouse, Rat . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1646Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-alpha + beta Synuclein antibody [EP1646Y] - BSA and Azide free (ab189217) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of alpha + beta Synuclein?
Anti-alpha + beta Synuclein [EP1646Y] - BSA and Azide free (ab189217) specifically detects a band for alpha + beta Synuclein (UniProt: P37840) at a molecular weight of 14kDa.
Other related products
We have a range of other formats of antibody clone [EP1646Y] also available for your convenience:
ab51252
, Carrier free - ab189217
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein and beta-synuclein are proteins implicated in the dynamics of neuronal function. Alpha-synuclein also known as SNCA is a synaptic protein weighing approximately 14 kDa expressed highly in the central nervous system. Unlike alpha-synuclein beta-synuclein shares a similar structure but differs in function and molecular weight. Both proteins belong to the synuclein family which also includes gamma-synuclein and are found primarily in pre-synaptic terminals contributing to synaptic vesicle physiology.
Biological function summary
Alpha- and beta-synuclein play roles in synaptic function and plasticity where they influence neurotransmitter release and vesicle recycling. These proteins do not generally form a stable complex with each other but their presence together suggests a regulatory interplay. Alpha-synuclein may promote vesicle formation and trafficking while beta-synuclein appears to counteract alpha's effects potentially protecting neurons from alpha-synuclein aggregation.
Pathways
Alpha- and beta-synuclein are key components in the synaptic vesicle cycle especially associated with the SNARE complex and neurotransmitter balance. Alpha-synuclein interacts with proteins like VAMP2 and STX1A in vesicle merging while beta-synuclein's regulatory role has implications in maintaining these interactions. In lipid metabolism alpha-synuclein influences fatty acid binding protein pathways impacting neuronal lipid transport and homeostasis.
Alpha- and beta-synuclein hold significant relevance particularly in neurodegenerative disorders like Parkinson's disease and dementia with Lewy bodies which show marked alpha-synuclein aggregates known as Lewy bodies. Alpha-synuclein's accumulation in neurons correlates with disease progression. Beta-synuclein on the other hand appears to have a protective role potentially mitigating the toxicity of alpha's aggregates. The relation between these synucleins underlines their importance in therapeutic research for these disorders.