Abcam, ab189246, Anti-Hepsin/HPN antibody [EP7654]

Size: 100µL / 1mL
Rabbit Monoclonal Hepsin/HPN antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP7654,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Product details:
This product has switched from a hybridoma to recombinant production method on 24th October 2023.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hepsin also known as HPN is a serine protease enzyme with a molecular weight of approximately 51 kDa. It is located on the cell surface and shows a high expression in hepatocytes and prostate tissue. Alternative names for this protein include HPN and hepto. Hepsin contains a trypsin-like domain and a transmembrane domain contributing to its proteolytic function. This enzyme plays an important role in cell growth and maintenance by cleaving other proteins.
Biological function summary
Hepsin functions in the activation of growth factors and proteolysis of the extracellular matrix. It does not form part of a large protein complex but cooperates with other proteases and factors to achieve its biological roles. Hepsin influences processes like cell migration tissue remodeling and metastatic spread highlighting its importance in cellular dynamics.
Pathways
Hepsin is involved in the activation of the HGF/MET signaling pathway. In this pathway Hepsin activates hepatocyte growth factor (HGF) which in turn interacts with the MET receptor to promote cellular behaviors such as proliferation and migration. This pathway shares connections with other proteases like matriptase which also partake in activating growth factors and modulating cellular activities.
Hepsin has connections to prostate cancer and liver fibrosis. In prostate cancer elevated levels of Hepsin may facilitate tumor progression by degrading extracellular matrix components enabling cancer cells to invade new tissues. Hepsin's activity links it to MET signaling which can enhance tumor growth. In liver fibrosis its role in modifying extracellular matrix proteins can influence fibrotic tissue development potentially interacting with proteins like TIMP1 which regulate matrix degradation.