Abcam, ab1898, Anti-TCR V beta 5 antibody [MEM-262]

Size: 100µg
Mouse Monoclonal TCR V beta 5 antibody. Suitable for Flow Cyt, IP, WB and reacts with Human samples. Cited in 6 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MEM-262,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:WB, IP, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Product details:
Functional ApplicationActivates T cells (V beta 5-related subset).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE., Storage buffer-pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TCR V beta 5 also known as T-cell receptor V beta 5 is a variable region of the T-cell receptor expressed on the surface of T lymphocytes. It consists of a protein chain that is part of the TCR complex with an approximate mass of around 29 kDa. You can find TCR V beta 5 specifically in T cells which are central components of the adaptive immune system. The expression of TCR V beta 5 allows T cells to recognize and bind to specific antigens presented by the Major Histocompatibility Complex (MHC) molecules on antigen-presenting cells.
Biological function summary
The variable region of the TCR V beta 5 plays a pivotal role in the immune system by contributing to the diversity of antigen recognition. The TCR V beta chain together with TCR alpha forms a heterodimer that is critical for the antigen specificity of T cells. TCR V beta 5 aids in the interaction with peptides bound to MHC molecules and helps to activate T-cell responses. It is an integral part of the TCR-CD3 complex composed of various chains that initiate signaling cascades essential for T-cell activation and function.
Pathways
TCR V beta 5 is involved in the T-cell receptor signaling pathway which is fundamental for T-cell activation differentiation and proliferation. The activation of TCR signaling involves key proteins like ZAP-70 and LAT that help propagate the signal downstream to initiate cellular responses. This pathway concurrently interacts with the MAPK/ERK pathway which further influences cell survival and proliferation. The interactions of these pathways highlight the important involvement of TCR V beta 5 in the adaptive immune response.
TCR V beta 5 relates to autoimmune conditions and oncological disorders. One such autoimmune disease connected to its function includes multiple sclerosis (MS) where an aberrant immune response mediates damage to the central nervous system. Additionally certain leukemias notably T-cell acute lymphoblastic leukemia show aberrant expression profiles of TCR components like TCR V beta 5. Relationships with proteins such as Lck and Fyn which are involved in signaling from the T-cell receptor highlight the implications of dysregulated TCR V beta 5 activity in these diseases.