Abcam, ab190060, HRP Anti-Hemagglutinin antibody

Size: 100µL
Chicken Polyclonal Hemagglutinin antibody. Suitable for ELISA, WB and reacts with Influenza A samples.
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Conjugation:HRP,
Carrier free:No,
Reacts with:Influenza A,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and the antibodies were conjugated to horseradish peroxidase (HRP)., Storage buffer-pH: 7Preservative: 0.002% Thimerosal (merthiolate)Constituents: 50% Glycerol (glycerin, glycerine), 0.6% HEPES, 0.29% Sodium chloride, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hemagglutinin also known as HA is a protein with a mass of approximately 70 kDa. This surface protein expresses mainly on the envelope of influenza viruses. Hemagglutinin plays an important role in binding the virus to host cell receptors and facilitates entry of viral particles into the target cells. The protein undergoes a conformational change to promote fusion between the viral envelope and host cell membranes which is essential for viral infectivity.
Biological function summary
Hemagglutinin mediates host immune recognition since it serves as a major antigenic component. The protein forms a trimeric complex with each subunit comprising two functional regions HA1 and HA2. HA1 is responsible for receptor binding while HA2 is involved in membrane fusion. The immunogenic nature of hemagglutinin challenges the immune system to produce antibodies targeting this structure making it a focus for vaccine design.
Pathways
Hemagglutinin directly interacts with the endocytosis pathway. This interaction facilitates the internalization of the virus into endosomes where the acidic environment triggers hemagglutinin-mediated fusion of viral and endosomal membranes. Hemagglutinin's function in these pathways closely relates to viral neuraminidase which aids in the release of progeny viruses from infected host cells after replication.
Hemagglutinin is notably central to influenza infection an acute respiratory condition with significant morbidity and mortality. Antigenic variations in hemagglutinin contribute to seasonal flu epidemics and potential pandemics. These variations also complicate vaccine formulation as antibodies generated against one hemagglutinin variant may be less effective against others. Additionally hemagglutinin maintains a relationship with the M2 ion channel protein which is important in viral uncoating and replication processes thereby influencing virus pathogenicity and disease outcomes.