Abcam, ab190374, Anti-Aurora A + B antibody [3H1]

Size: 100µL
Mouse Monoclonal Aurora A antibody. Suitable for WB, ICC/IF and reacts with Human, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AURKA.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3H1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human AURKA.O14965,
Specificity:ab190374 was tested for binding to expressed human Aurora A, B and C and shown to react with both Aurora A and B, but not C.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aurora A and Aurora B are serine/threonine kinases also known as STK6 and STK12 respectively. They play a central role in cell division by controlling important processes like centrosome maturation and cytokinesis. Aurora A has a molecular mass of approximately 46 kDa while Aurora B is around 39 kDa. These proteins are expressed in proliferating tissues and have increased expression in cancer cells. Their expression patterns suggest a role in regulating cell cycle progression. The activity of Aurora A and B is cell cycle-dependent peaking during mitosis.
Biological function summary
Aurora kinases serve as regulators of mitotic events to ensure proper chromosome segregation. Aurora A is often associated with the centrosome and mitotic spindle while Aurora B part of the chromosomal passenger complex functions at the centromeres and midzone of dividing cells. They work together to execute processes such as spindle assembly and the correction of incorrect kinetochore-microtubule attachments. This coordination is essential for preserving genomic stability across cell divisions.
Pathways
Aurora A and B kinases function in the regulation of the mitotic checkpoint and spindle assembly pathway. They interact closely with proteins such as PLK1 and BUB1B which are involved in ensuring accurate chromosome alignment and separation. Aurora A's involvement in centrosome maturation links it to pathways regulating cell polarity while Aurora B's role in cytokinesis connects it to pathways overseeing cleavage plane determination and contractile ring formation. These pathways integrate signals from cell division regulators and communicate with other checkpoint proteins to coordinate orderly mitosis.
Dysregulation of Aurora kinases has a strong link to various cancers given their role in chromosome segregation and cell cycle control. Overexpression or mutations in Aurora A and B frequently occur in breast cancer and colorectal cancer contributing to oncogenic transformation and tumor progression. Their aberrant activity results in aneuploidy and mitotic defects often co-occurring with alterations in proteins like TP53 a well-known tumor suppressor. This relationship with cancer highlights the significance of Aurora kinases as potential therapeutic targets in oncology research and drug development.