Abcam, ab190467, Anti-alpha-hemolysin antibody [8B7] - N-terminal

Size: 50µg
Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) is a mouse monoclonal antibody detecting alpha-hemolysin in Western Blot, Neut . Suitable for Staphylococcus aureus . - Over 10 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8B7,
Isotype:IgG3,
Light chain type:kappa,
Carrier free:No,
Reacts with:Staphylococcus aureus,
Applications:FuncS (Neut/Block), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Staphylococcus aureus hly. The exact immunogen used to generate this antibody is proprietary information.P09616,
Specificity:ab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region.

Product details:
What is this antibody validated in?
Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Neutralising in Staphylococcus aureus samples.
What is the molecular weight of alpha-hemolysin?
Anti-alpha-hemolysin [8B7] - N-terminal (ab190467) specifically detects a band for alpha-hemolysin (UniProt: P09616) at a molecular weight of 36kDa.
Trusted by the scientific community
Anti-alpha-hemolysin [8B7] - N-terminal (ab190467) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-hemolysin also known as alpha-toxin or alpha-haemolysin is a 33.2 kDa pore-forming toxin produced by Staphylococcus aureus. This toxin forms heptameric transmembrane pores with each unit contributing to the overall function of the complex. These pores disrupt target cell membranes leading to hemolysis by osmotic lysis. Alpha-hemolysin is predominantly expressed in S. aureus strains and plays a significant role in bacterial pathogenicity.
Biological function summary
The activity of alpha-hemolysin disrupts plasma membranes of host cells causing leakage of essential ions and metabolites. This action triggers host cell death through destruction of cell integrity. As a monomer alpha-hemolysin assembles into a homo-heptameric structure on the surface of the target membrane creating a channel that allows uncontrolled ion passage. This activity is an important part of the infection process by S. aureus compromising host cell viability and facilitating bacterial invasion.
Pathways
Alpha-hemolysin is integral to pathogenesis pathways notably the Staphylococcus aureus infection pathway. This toxin works in conjunction with other virulence factors to enhance bacterial survival and proliferation. It interacts with other proteins and surface receptors that modulate immune evasion and tissue colonization such as fibronectin-binding proteins influencing disease progression and immune response.
Alpha-hemolysin contributes to conditions like pneumonia and skin abscesses caused by Staphylococcus aureus infections. The toxin's ability to damage host tissues and immune cells helps pathogenic bacteria establish infections. It is often associated with other toxins such as leukocidins which together enhance the bacterium's capacity to persist in hostile environments and evade host defenses.