Abcam, ab190533, Monkey Albumin ELISA Kit

Size: 1 x 96Tests
Monkey Albumin ELISA Kit is a sandwich ELISA used to quantify Monkey Albumin with a sensitivity of 1.795 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range – quantifies 12.5 – 400 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum,
Reacts with:Monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.795 ng/mL,
Range:12.5 - 400 ng/mL,
Assay Platform:Microplate

Product details:
Monkey Albumin ELISA Kit ab190533 is a sandwich ELISA kit to measure Monkey Albumin in Serum, Plasma, and Urine with a sensitivity of 1.795 ng/mL.
How the assay works
Monkey Albumin ELISA Kit ab190533 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Monkey Albumin ELISA Kit ab190533 protocol summary:
1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.
3. Add TMB Solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.
Albumin (Alb) is a polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.
The calibrator used in this kit is of human origin. It is quantitated with purified Cynomolgus monkey albumin and behaves identically with it.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.