Abcam, ab190691, Anti-Ikaros antibody [EPR13791] - C-terminal

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Ikaros antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13791,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Based on the sequence analysis, ab190691 recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.This product is not suitable for Mouse in WB, ICC/IF or IP.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.
Biological function summary
Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.
Pathways
The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.
Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.