Abcam, ab191634, Recombinant human B7H4 protein

Size: 100µg
Recombinant human B7H4 protein is a Human Fragment protein, in the 29 to 258 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its ability to inhibit anti-CD3 antibody induced IL2 secretion in Human T lymphocytes.The ED50 for this effect is typically 0.75 - 3 μg/ml.,
Accession:Q7Z7D3,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute with sterile deionized water to a concentration of 700 µg/ml.,
Storage buffer:pH: 7.4Constituents: PBS, 5% Trehalose

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.
Biological function summary
B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.
Pathways
B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.
B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.