Abcam, ab191825, Biotin Anti-Glucagon antibody [08]

Size: 25µg
Mouse Monoclonal GLP-1 antibody - conjugated to Biotin. Suitable for sELISA and reacts with Rat, Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GCG.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:08,
Isotype:IgG1,
Light chain type:kappa,
Conjugation:Biotin,
Carrier free:No,
Reacts with:Rat, Human, Pig,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GCG. The exact immunogen used to generate this antibody is proprietary information.P01275

Properties and Storage Information:
Form-Liquid, Purification notes-ab191825 is Protein A purified, before biotinylation., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: 99% Phosphate Buffer, 0.81% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucagon also known as the “hunger hormone" is a 29-amino acid peptide hormone with a mass of approximately 3.5 kDa. It plays an important role in glucose metabolism and regulation of blood sugar levels. The human pancreas specifically the alpha cells located in the islets of Langerhans synthesizes and secretes glucagon. This hormone binds to glucagon receptors which are widely expressed across tissues including the liver and kidney where it initiates a cascade of signaling events.
Biological function summary
Glucagon plays a central role in maintaining glucose homeostasis. It increases blood glucose by promoting gluconeogenesis and glycogenolysis in the liver. Though glucagon mainly acts independently it exhibits significant interactions with other metabolic hormones such as insulin. This interaction helps balance blood sugar levels as glucagon and insulin work in opposition to ensure optimal blood glucose regulation.
Pathways
Glucagon primarily impacts the cAMP signaling pathway significantly increasing intracellular cAMP levels. This pathway initiates a complex series of downstream events including increased enzyme activity for gluconeogenesis and glycogen breakdown in the liver. Glucagon also cross-talks with insulin signaling pathways enabling the intertwined regulation of metabolism through these hormones and maintaining glucose balance.
Glucagon is closely linked to diabetes and hyperglycemia. Individuals with diabetes often exhibit dysregulated glucagon secretion leading to unstable blood sugar levels. Glucagon interacts with insulin in the pathology of diabetes where an improper balance between these hormones can exacerbate the disease. The understanding of glucagon's role in these conditions makes it a target for new therapeutic strategies including recombinant glucagon treatments and assays.