Abcam, ab191851, Anti-PDILT antibody

Size: 100µL
Rabbit Polyclonal PDILT antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IP, IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. Database linkQ9DAN1

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-pH: 6 - 8.5Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDILT also known as Protein Disulfide Isomerase-Like Testis Expressed is a member of the protein disulfide isomerase (PDI) family. This protein has a molecular mass of approximately 70 kDa and a unique structure. PDILT lacks one of the two active CGHC motifs usually seen in this family while it possesses the ER-retention signal KDEL. This protein is expressed mainly in the testis with a strong association to male germ cells. Its expression links to the endoplasmic reticulum suggesting a role in protein folding and quality control.
Biological function summary
PDILT plays an essential role in ensuring proper sperm development. Although it is not formally part of a larger protein complex it interacts with other proteins in the testis to facilitate the assembly of protein disulfide bonds. These interactions ensure the correct formation of complex disulfide-bonded proteins which are necessary for sperm structure and function. PDILT is involved in managing oxidative stress within the cells during the process of spermatogenesis.
Pathways
PDILT plays an important part in the oxidative protein folding pathway within the endoplasmic reticulum. This pathway is critical for forming disulfide bonds in proteins a process important for their stability and function. PDILT has connections with proteins like Calnexin an important component of the quality control pathway in the ER. Its functional synergy with other PDI family members highlights its role in maintaining the balance of protein homeostasis during reproduction.
PDILT has shown links to male infertility particularly regarding improper sperm motility or structure. Its malfunction can lead to a disruption in the formation of sperm disulfide bonds resulting in structural abnormalities. This dysfunction associates with other proteins like Calreticulin which is also involved in proper protein folding. Current research continues to investigate PDILT's broader implications in fertility emphasizing its significance in maintaining reproductive health.