Abcam, ab192033, Anti-Nectin-4 antibody [EPR15613-68]

Size: 20µL / 100µL / 1mL
Anti-Nectin-4 antibody [EPR15613-68] (ab192033) is a rabbit monoclonal antibody detecting Nectin-4 in Western Blot, IHC-P . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15613-68,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Customers should note, the current antibody has been examined with alternative automated IHC staining platforms and optimal results were obtained using the Ventana DISCOVERY ULTRA with associated kits and reagents. We were not able to obtain optimal results with Leica BOND platform when using the Polymer Refine detection kit, 20min ER1 antigen retrieval and 16min antibody incubation at room temperature. Further optimisation may be needed when using the Leica BOND platform.The antibody detected a 50kDa and 70kDa non-specific band in the negative sample MDA-MB-231, which are close to the target band. Therefore, we recommend including a negative sample when performing Western Blot analysis.We suggest using the whole cell and cell supernatant lysate side by side to observe the expression of different form of Nectin-4. The cell supernatant can detect soluble Nectin-4 mainly (PMID: 15784625).Negative control: MDA-MB-231 (15784625, PMID: 31617074, PMID: 24386110, PMID: 17474988).

Product details:
Product Specifications
Anti-Nectin-4 antibody [EPR15613-68] (ab192033) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human samples.
Anti-Nectin-4 antibody [EPR15613-68] (ab192033) specifically detects Nectin-4 (UniProt ID: Q96NY8; Molecular weight: 52kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Nectin-4 antibody [EPR15613-68] (ab192033) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Nectin-4 antibody [EPR15613-68] (ab192033) has been cited over 14 times in peer reviewed journals and is trusted by the scientific community.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle