Abcam, ab192243, Anti-NDUFB4 antibody [EPR16240]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal NDUFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16240,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFB4 also known as NADH: ubiquinone oxidoreductase subunit B4 plays a role in the mitochondrial respiratory chain complex I. Weighing approximately 15 kDa NDUFB4 is expressed in mitochondria across various cell types. It couples electron transfer from NADH to ubiquinone thereby contributing to energy production process in cells. NDUFB4 is important in maintaining mitochondrial functionality due to its role in the electron transport chain.
Biological function summary
NADH: ubiquinone oxidoreductase subunit B4 is part of the NADH dehydrogenase complex I. This complex is involved in the initial step of the mitochondrial electron transport chain. NDUFB4 supports the bioenergetic functions of cells by facilitating redox reactions. It also helps establish the electrochemical gradient needed for ATP synthesis. This protein interacts synergistically with other subunits in complex I to ensure efficient energy conversion.
Pathways
NADH dehydrogenase complex I which includes NDUFB4 is central in oxidative phosphorylation. This process supports energy production by converting NADH and FADH2 into ATP. NDUFB4 is integrally linked with proteins like NDUFS2 and NDUFV1 within this pathway ensuring electron transport is seamless. Complex I contributes to metabolic processes like the TCA cycle by feeding the mitochondria with necessary energy intermediates.
Malfunction of NDUFB4 in complex I links to mitochondrial diseases such as Leigh syndrome. This syndrome affects central nervous system development. Mitochondrial encephalopathies are another group where NDUFB4 involvement is observed. In these conditions proteins like NDUFA1 and mitochondrial DNA mutations can manifest similar neurodegenerative symptoms due to impaired oxidative phosphorylation. Therefore understanding NDUFB4’s function helps in identifying therapeutic interventions for these disorders.