Abcam, ab192890, Anti-LC3B antibody [EPR18709] - Autophagosome Marker

Size: 20µL / 100µL / 1mL
Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) is a rabbit monoclonal antibody detecting LC3B in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 460 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18709,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product.

Product details:
Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P and WB.
Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) was first used in a scientific publication in 2017 and has been cited over 468 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) has been confirmed by Western Blot testing in LC3B knockout HepG2 cells (ab277828).
Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) has 17 independent reviews from customers.
Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890) specifically detects LC3B (UniProt ID: Q9GZQ8; Molecular weight: 15kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR18709 -
ab221794
Antibody clone EPR18709 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
ab225382
ab225383
ab307768
LC3B is a protein involved in autophagy, a process that helps maintain cellular homeostasis by degrading damaged components. In oncology, LC3B is used as a marker to assess autophagy levels in cancer cells, which can influence tumor progression and response to therapy.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LC3B also known as Microtubule-associated protein 1A/1B-light chain 3 is a core protein in autophagy processes. LC3B protein has a molecular weight of approximately 14 kDa. Researchers often utilize LC3B as a marker to study autophagic activity especially through LC3B immunofluorescence and LC3B Western blot analyses. This protein is predominantly found in the cytoplasm and is widely expressed in various tissues with higher expression in tissues undergoing high turnover or stress.
Biological function summary
LC3B contributes to the formation of autophagosomes playing an essential role in autophagy. During autophagosome formation LC3B conjugates with phosphatidylethanolamine which localizes it to autophagosome membranes. This lipidated form is important for the elongation and closure of autophagosomes. LC3B is also a component of the autophagy-related protein complex collaborating with other Atg proteins to ensure the proper degradation of cytoplasmic contents.
Pathways
LC3B plays an important role in the autophagy pathway important for cellular homeostasis and stress response. This pathway interfaces with the mTOR signaling pathway where LC3B helps regulate mTOR activity by controlling autophagosome dynamics. LC3B is associated with proteins like Beclin-1 and p62/SQSTM1 within these pathways coordinating the sequestration and degradation of proteins and organelles.
LC3B is implicated in cancer and neurodegenerative diseases. Altered LC3B levels and function are observed in cancers linking this protein to tumorigenesis through its role in autophagy. In neurodegenerative disorders like Parkinson's disease LC3B interacts with proteins such as alpha-synuclein influencing the accumulation of protein aggregates and neuronal health. The modulation of LC3B activity is of interest in therapeutic strategies targeting these diseases.