Abcam, ab193468, Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [EPR18855]

Size: 20µL / 100µL / 1mL
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [EPR18855] (ab193468) is a rabbit monoclonal antibody for Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP, Dot Blot . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18855,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ChIP, IP, ICC/IF, Flow Cyt (Intra), IHC-P, Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [EPR18855] (ab193468) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of RNA polymerase II CTD repeat YSPTSPS?
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) [EPR18855] (ab193468) specifically detects a band for RNA polymerase II CTD repeat YSPTSPS (UniProt: P04050) at a molecular weight of 192kDa.
Trusted by the scientific community
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) [EPR18855] (ab193468) was first used in a scientific publication in 2015 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR18855] also available for your convenience: ab193468, Alexa Fluor® 488 -
ab237279
, Alexa Fluor® 647 -
ab237280
, PE -
ab237281
, Carrier free -
ab238450
, APC -
ab310840
, Alexa Fluor® 594 -
ab311644
, Alexa Fluor® 568 -
ab312917
, Alexa Fluor® 555 -
ab313129
, Alexa Fluor® 750 -
ab321332
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA polymerase II CTD repeat YSPTSPS also known as the C-terminal domain of RNA polymerase II is a critical component of the RNA polymerase II enzyme commonly referred to as pol II. This domain is characterized by the repetitive sequence YSPTSPS which plays a significant role in the regulation of transcription. The mass of RNA polymerase II including its CTD varies but is essential for its function in gene expression. RNA polymerase II with the CTD is expressed in the nucleus of eukaryotic cells where it orchestrates the transcription of DNA into mRNA.
Biological function summary
RNA polymerase II CTD repeat YSPTSPS is essential for the transcription progression from initiation to termination. It is part of the large RNA polymerase II complex interacting with various transcription factors and enzymes necessary for RNA processing. The phosphorylation state of the CTD particularly on serine residues regulates interactions with splicing machinery and other RNA processing factors. This modulation ensures the coupling between transcription and RNA processing events controlling mRNA synthesis and maturation.
Pathways
RNA polymerase II CTD repeat YSPTSPS is important in the mRNA synthesis pathway specifically in transcriptional regulation and processing of nascent RNA transcripts. It interacts with proteins such as the transcription factors TFIIH and TFIIB which aid in promoter recognition and open complex formation. The CTD's dynamic phosphorylation pattern allows integration into multiple cellular pathways most importantly connecting transcription with RNA splicing and transport pathways.
Abnormal function or mutations in RNA polymerase II CTD repeat YSPTSPS associate with diseases such as transcription-related syndromes and certain cancers. Deficient CTD phosphorylation can lead to improper mRNA processing resulting in neural developmental disorders. Additionally its interaction with proteins like CDK7 which phosphorylates the CTD links it to tumors where transcriptional dysregulation is a hallmark. Understanding the CTD's role in these diseases provides insight into therapeutic targets and strategies for intervention.