Abcam, ab193634, Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody

Size: 100µL
Rabbit Polyclonal Ephrin B1 phospho Y324 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EFNB2 phospho Y311.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human EFNB1 phospho Y324 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. Database linkP98172The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human EFNB3 phospho Y318. Database linkQ15768,
Specificity:ab193634 detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318 respectivly.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab193634 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ephrin B1 Ephrin B2 and Ephrin B3 also known as EFNB1 EFNB2 and EFNB3 are transmembrane proteins involved in cell communication. These proteins belong to the ephrin family and typically exhibit molecular masses ranging from 25 to 30 kDa. They are predominantly expressed in the nervous system vasculature and various tissues such as bones and liver. By binding to Eph receptors which are a subclass of receptor tyrosine kinases ephrins play a major role in bidirectional signaling between adjacent cells.
Biological function summary
These ephrins operate together with Eph receptors in a larger signaling complex. They influence cellular processes like boundary formation neural development and angiogenesis. Their interaction dictates the positioning and migration of cells during development. Ephrin B2 and Ephrin B3 specifically guide the formation of neural pathways and support vascularization while Ephrin B1 is important for craniofacial development. The dynamic interactions within these complexes highlight their regulatory role across various stages of organismal development.
Pathways
These ephrins and Eph receptors are integral to the Eph/ephrin signaling pathway and the MAPK signaling pathway. The Eph/ephrin pathway governs cellular repulsion and attraction mechanisms critical for tissue patterning. Ephrin B2 and Ephrin B3 through their interaction with EphB receptors influence these signaling cascades. In the MAPK pathway their activity affects cell proliferation and differentiation by interacting with proteins like RAS and RAF which contribute to signaling cascades that impact cell fate decisions.
These ephrins are associated with developmental defects and cancer. Mutations or dysregulation in Ephrin B1 can lead to craniofrontonasal syndrome characterized by abnormal facial and skeletal development. Ephrin B2 and Ephrin B3 have connections to tumor growth and metastasis often associated with aberrant angiogenesis. Interplay with proteins like VEGF in tumor environments highlights their role in promoting aberrant vascular networks contributing to cancer progression.