Abcam, ab193710, Human NOV ELISA Kit (Nephroblastoma Overexpressed)

Size: 1 x 96Tests
Human NOV ELISA Kit (Nephroblastoma Overexpressed) is a Sandwich (quantitative) ELISA for the measurement of Human NOV (Nephroblastoma Overexpressed) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 pg/mL,
Range:2 - 30000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Abcam's NOV (Nephroblastoma Overexpressed) Human ELISA Kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of human NOV in serum, plasma, and cell culture supernatant.
This assay employs an antibody specific for human NOV coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures NOV present in the samples. The wells are washed and biotinylated anti-human NOV antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of NOV bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCN3 also known as NOV (Nephroblastoma Overexpressed) is a protein that has important roles in cellular processes. This protein belongs to the CCN family of proteins with an approximate molecular mass of 34-38 kDa. CCN3 is expressed in various tissues including kidney heart and brain reflecting its involvement in diverse physiological activities. It is particularly noted for its role in tissue development and repair processes.
Biological function summary
CCN3 influences several aspects of cell behavior including proliferation differentiation and migration. It does not function as part of a larger complex but acts as an individual entity facilitating communication between cells and the extracellular matrix. CCN3 also affects angiogenesis a critical process in both health and disease by modulating vascular endothelial growth factor (VEGF) pathways therefore impacting blood vessel formation and maintenance.
Pathways
CCN3 interacts primarily with key signaling pathways such as Wnt and TGF-beta signaling. Within the Wnt pathway CCN3 serves as a regulatory component modulating the activity of other proteins involved in cell growth. Similarly in the TGF-beta pathway it contributes to the regulation of cellular proliferation and differentiation by affecting interactions with other proteins like SMADs which are signal transducers in TGF-beta signaling.
CCN3 has connections to conditions like nephroblastoma often referred to as Wilms Tumor and fibrosis. In nephroblastoma altered expression of CCN3 correlates with tumor progression and metastasis. It interacts with proteins like WNT1 influencing tumor growth and development. In fibrosis which affects organs such as the liver and lungs CCN3’s modulation of TGF-beta signaling plays a significant role impacting fibrogenesis through its interaction with ECM components.