Abcam, ab193714, Human Serpin A4 ELISA Kit

Size: 1 x 96Tests
Human Serpin A4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Serpin A4 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.12 ng/mL,
Range:0.12 - 35 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Abcam's Serpin A4 Human ELISA Kit (ab193714) is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of human Serpin A4 in serum, plasma, and cell culture supernatant.
This assay employs an antibody specific for human Serpin A4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Serpin A4 present in the samples. The wells are washed and biotinylated anti-Human Serpin A4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Serpin A4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Kallistatin also known as PI-4 is a serine proteinase inhibitor with a mass of approximately 52 kDa. You find it mainly in the liver as part of the serpin superfamily. This protein acts mechanically by inhibiting the tissue kallikrein-kinin system which involves proteolytic processes that regulate vascular homeostasis and blood pressure. In addition to the liver kallistatin is expressed in endothelial cells and various human tissues.
Biological function summary
Kallistatin participates in inhibiting angiogenesis and inflammation impacting cellular behavior and responses. As a serpin it functions uniquely in forming stable complexes with target enzymes like tissue kallikrein. Through this mechanism kallistatin contributes to maintaining vascular integrity and preventing excessive vascular growth or inflammation that could lead to pathology.
Pathways
The inhibitory effects of kallistatin integrate into the kallikrein-kinin system and the renin-angiotensin system. In the kallikrein-kinin system kallistatin regulates processes involving vascular tone and fluid balance. It interacts with proteins such as bradykinin mediating inflammatory responses and endothelial cell function through these key pathways.
Researchers link kallistatin with conditions like hypertension and cancer. Its role in inhibiting angiogenesis and inflammation helps understand its protective effects against tumor growth and metastasis. In hypertension kallistatin's relationship with the renin-angiotensin system connects it to other proteins like angiotensin II influencing blood pressure regulation and vascular health.