Abcam, ab193716, Human Thrombospondin 1 ELISA Kit

Size: 1 x 96Tests
Human Thrombospondin 1 ELISA Kit is a sandwich ELISA designed to quantify Human Thrombospondin 1 with a sensitivity of 6 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 6 - 2000 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 6 ng/mL,
Range:6 - 2000 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Thrombospondin 1 ELISA Kit ab193716 is a sandwich ELISA to measure Human Thrombospondin 1 in serum, plasma, cell culture supernatant with a sensitivity of 6 ng/ml.
How the assay works
This assay employs an antibody specific for Human Thrombospondin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Thrombospondin 1 present in the samples. The wells are washed and biotinylated Human Thrombospondin 1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Thrombospondin 1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Thrombospondin 1 ELISA Kit ab193716 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Thrombospondin 1 also known as TSP1 is a multi-functional glycoprotein involved in cellular processes like adhesion migration and tumorigenesis. It has a molecular weight of approximately 450 kDa. TSP1 is produced by multiple cell types including endothelial cells and fibroblasts and it is widely expressed in the extracellular matrix of both normal and tumor tissues. As a member of the thrombospondin family it interacts with cell receptors like integrins and CD36 to mediate its diverse functions.
Biological function summary
TSP1 is significant in modulating angiogenesis immune response and apoptosis. It acts as an anti-angiogenic factor by inhibiting endothelial cell proliferation and migration. TSP1 is a part of a larger complex where it interacts with other matrix proteins and growth factors to fine-tune cellular behavior within tissues. Through its binding domains it inhibits matrix metalloproteinases helping regulate matrix composition and tissue remodeling.
Pathways
TSP1 influences both the TGF-beta and NF-kB signaling pathways. In the TGF-beta pathway TSP1 activates latent TGF-beta contributing to tissue repair and fibrosis processes. It works synergistically with proteins like SMADs in these pathways. In the NF-kB pathway TSP1 modulates immune responses and inflammatory processes. Its interaction with CD36 and integrins in these pathways underlines its versatile role in cellular signaling.
TSP1 has been investigated in cancer progression and cardiovascular diseases. In cancer TSP1's anti-angiogenic properties suppress tumor vascularization which can slow tumor growth. Aberrant TSP1 expression levels have also been linked to atherosclerosis development as it interacts with endothelial cells and inflammatory molecules like TNF-alpha. In both these contexts TSP1 engages with proteins such as integrins and CD36 to exert its influence on disease progression.