Abcam, ab193722, Mouse Endocan ELISA Kit

Size: 1 x 96Tests
Mouse Endocan ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Endocan in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.1 ng/mL,
Range:0.1 - 30 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Abcam's Endocan Mouse ELISA Kit (ab193722) is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of mouse Endocan in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Endocan coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Endocan present in the samples. The wells are washed and biotinylated anti-mouse Endocan antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Endocan bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Endothelial cell-specific molecule 1 (ESM1) also known as endocan is a proteoglycan with a mass of approximately 50 kDa. It is primarily expressed in endothelial cells of blood vessels particularly in the lungs and the kidneys. ESM1 consists of a core protein covalently attached to dermatan sulfate chains. Its expression often increases under inflammatory conditions which highlights its role in vascular biology.
Biological function summary
ESM1 acts in the regulation of cell adhesion and migration processes. It interacts with leukocytes and participates in modulating inflammatory responses. ESM1 does not form part of a multi-protein complex but its activity can influence the behavior of other cell surface molecules and receptors. This function positions ESM1 as a critical player in the dynamics of vascular endothelium.
Pathways
ESM1 modulates inflammation and angiogenesis by participating in pathways involving vascular endothelial growth factor (VEGF). It interacts with proteins such as VEGF receptor-2 impacting endothelial permeability and new blood vessel formation. These interactions situate ESM1 within the signaling networks that regulate endothelial function and vascular homeostasis.
Research associates ESM1 with conditions such as sepsis and cancer. High levels of this molecule correlate with poor prognosis in these diseases linking it functionally to VEGF and other factors involved in abnormal endothelial cell activity. Understanding ESM1's role provides insights into mechanisms of disease progression and potential therapeutic targets.