Abcam, ab194299, Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal EHMT1/GLP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18667,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse, Human,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EHMT2 also known as G9A and EHMT1 also known as GLP are histone methyltransferases. These enzymes are responsible for catalyzing the methylation of histone H3 on lysine 9 (H3K9) leading to transcriptional repression. G9A has an approximate molecular mass of 126 kDa. Both G9A and GLP are expressed in various tissues with high levels in the brain heart and skeletal muscle indicating their important function in these areas.
Biological function summary
G9A and GLP play an important role in chromatin modification and gene silencing. They usually exist as part of a complex which includes other proteins such as WIZ and CDYL. They function together to establish and maintain the repressive chromatin state influencing cellular processes like differentiation and development. The presence of G9A/GLP in the transcriptional regulation impacts various cell-specific functions and contributes to the control of cellular proliferation.
Pathways
G9A and GLP are integral to the epigenetic regulatory pathways. They play a major role in the gene silencing pathway by cooperating with HP1 proteins to enhance chromatin compaction. One important pathway involves G9A contributing to the growth factor signaling pathway affecting the expression of genes fundamental to cell growth and survival. G9A and GLP interaction with DNA methylation pathways makes them associate with DNMT1 further enriching their regulatory potential.
G9A and GLP have associations with cancer and neurological disorders. In cancer G9A affects tumor growth and survival due to its role in gene silencing of tumor suppressor genes and it often connects with EZH2 in the disease context. In neurological disorders particularly neurodevelopmental conditions such as Kleefstra syndrome EHMT1 mutations impact GLP linking it to altered brain function and development. The dysregulation of these proteins underpins their significance in these pathological states making them potential targets for therapeutic intervention.