Abcam, ab1944, Anti-Lambda Free Light Chain antibody [3D12]

Size: 500µg
Mouse Monoclonal Lambda Free Light Chain antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3D12,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.

Product details:
Concentration varies from lot to lot and can be provided on request.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purity tested by electrophoresis., Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lambda Free Light Chain also known as lambda light chain or lambda FLC represents an essential component of immunoglobulin molecules. These polypeptide chains typically have a molecular weight of approximately 22.5 kDa. Lambda free light chains are expressed by B cells in the bone marrow and lymphoid tissues. They eventually appear in the bloodstream where they circulate as part of the broader immune response.
Biological function summary
Free lambda light chains contribute to the assembly of immunoglobulin molecules by forming dimers with heavy chains. They participate in the adaptive immune system's ability to identify and neutralize pathogens. Lambda FLCs operate as part of immunoglobulin G (IgG) complexes which enhance immune response efficiency. When synthesized in excess they are secreted into the bloodstream helping mediate various immune functions.
Pathways
Lambda FLC plays a role in the antibody-mediated immune response and complement activation pathways. In these pathways lambda light chains interact with other immunoglobulin molecules to initiate pathogen opsonization and clearance. Connections with proteins like immunoglobulin heavy chain exemplify their involvement in complex immune cascades that defend the host against infections.
An elevated level of lambda free light chains often indicates multiple myeloma or light chain amyloidosis. In multiple myeloma abnormal plasma cells produce excess lambda FLC disrupting normal blood cell production. The condition associates with proteins such as Bence Jones proteins composed of excess light chains that can be detected in urine and serve as important diagnostic markers.