Abcam, ab195352, Anti-METTL3 antibody [EPR18810]

Size: 20µL / 100µL / 1mL
Anti-METTL3 antibody [EPR18810] (ab195352) is a rabbit monoclonal antibody detecting METTL3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Clone EPR18810 is the most cited clone to METTL3 - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 290 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18810,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, Flow Cyt (Intra), IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-METTL3 antibody [EPR18810] (ab195352) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Anti-METTL3 antibody [EPR18810] (ab195352) was first used in a scientific publication in 2016 and has been cited over 293 times in peer reviewed journals. It's performance in Western Blot and IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-METTL3 antibody [EPR18810] (ab195352) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-METTL3 antibody [EPR18810] (ab195352) has been confirmed by Western Blot testing in METTL3 knockout Mouse embryonic stem cells cells.
Anti-METTL3 antibody [EPR18810] (ab195352) has 8 independent reviews from customers.
Anti-METTL3 antibody [EPR18810] (ab195352) specifically detects METTL3 (UniProt ID: Q86U44; Molecular weight: 65kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR18810 -
ab221795
Antibody clone EPR18810 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
ab216936
ab217109
Highly-cited antibody with >400 citations. A highly specific KO validated METTL3 antibody, essential for studying RNA methylation and mRNA splicing. This antibody is crucial in epigenetic regulation research, particularly in understanding METTL3's role in gene expression and cancer progression. It is widely used in studies of METTL3 inhibitors and the METTL3-METTL14 complex. Elevated METTL3 expression is linked to cancer progression, particularly in lung adenocarcinoma, while WTAP overexpression is associated with increased cell migration and invasion in various cancers.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The METTL3 protein also known as methyltransferase like 3 plays an important role in the modification of RNA. It is a component of the N6-adenosine-methyltransferase complex and is responsible for introducing the m6A mark on RNA. The molecular weight of METTL3 is approximately 70 kDa. This protein shows high expression in the human central nervous system and various cancer tissues indicating its broad significance.
Biological function summary
METTL3 contributes to the post-transcriptional regulation of gene expression by modulating m6A methylation. It functions as part of a complex with METTL14 and Wilms' tumor 1-associating protein (WTAP) to execute this modification. The m6A mark influences RNA stability splicing export and translation affecting cellular processes significantly.
Pathways
METTL3 operates within critical pathways such as the mRNA stability and processing pathway. In these pathways METTL3 interacts with proteins like METTL14 and YTH domain-containing proteins which are important for m6A reader function. Together they regulate RNA processing events that influence cellular function and adaptation.
METTL3 associates strongly with cancer development and neurological disorders. In cancers alterations in METTL3 expression affect tumor progression and metastasis impacting oncogenes like MYC. In the realm of neurological disorders METTL3's role in m6A modification affects RNA regulation aligning it with proteins like FTO which also participate in m6A demethylation processes. Understanding these connections highlights METTL3 as a potential target for therapeutic interventions.