Abcam, ab195461, Human Complement C3b ELISA Kit

Size: 1 x 96Tests
Human Complement C3b ELISA Kit is a sandwich ELISA designed to quantify Human Complement C3b with a sensitivity of 67 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range - quantifies 0.3 - 1.1 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 67 pg/mL,
Range:0.156 - 5 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human Complement C3b ELISA Kit ab195461 is a sandwich ELISA to measure Human Complement C3b in serum, plasma, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 67 pg/ml.
How the assay works
A Complement C3b specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3b specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3b captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Complement C3b ELISA Kit ab195461 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3 also known as complement C3 or anti C3b is a central protein in the complement system with an approximate molecular mass of 187 kDa. This protein exists in blood plasma as an inactive precursor and is produced mainly in the liver. C3 gets activated through cleavage into two fragments C3a and C3b. The functional fragment C3b covalently attaches to pathogen surfaces through a process called opsonization allowing it to 'tag' pathogens for destruction by immune cells.
Biological function summary
Complement C3b plays an integral role in innate immunity. C3b is a part of the complement cascade forming complexes such as C3bBb which acts as a convertase in the amplification of the complement response. Through opsonization C3b promotes the binding and uptake of pathogens by phagocytes therefore enhancing immune defense.
Pathways
C3b integrates into the alternative and classical complement pathways. In these pathways C3b binds with factors like B and D contributing to the formation of the C5 convertase and progressing to the membrane attack complex. These interactions involve proteins such as C5 and Factor B essential for the effective destruction of pathogens and the clearance of immune complexes.
Complement C3b shows associations with diseases involving improper immune regulation such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Mutations affecting C3b's interactions with regulatory proteins such as Factor H can lead to uncontrolled complement activation which contributes to the pathology of these diseases. Understanding these interactions provides insights into therapeutic targets for managing complement-related diseases.