Abcam, ab196355, Anti-Ryanodine receptor 2/RYR-2 antibody - N-terminal

Size: 100µg
Rabbit Polyclonal Ryanodine receptor 2/RYR-2 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RYR2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RYR2. The exact immunogen used to generate this antibody is proprietary information.Q92736

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ryanodine receptor 2 (RYR-2) also known as cardiac ryanodine receptor is an intracellular calcium release channel that plays an important role in cardiac muscle contraction. This large protein has a mass of about 564 kDa and is mainly expressed in cardiac muscle tissue. Mechanically RYR-2 regulates the release of calcium ions from the sarcoplasmic reticulum into the cytosol. This calcium release occurs through the calcium-induced calcium release mechanism which is important in excitation-contraction coupling in cardiac muscle cells.
Biological function summary
RYR-2 enables the heart muscle cells to contract and relax by supplying calcium ions necessary for muscle contraction. It is part of a larger complex that includes proteins such as FK506-binding protein 12.6 (FKBP12.6) and calsequestrin which help regulate its function. Proper function of this complex is necessary for maintaining the rhythmic contraction and relaxation cycles of the heart. Any disruptions in this function can lead to irregular heartbeats.
Pathways
RYR-2 is significant in cardiac calcium signaling pathways that involve calcium-induced calcium release. Through this pathway it works closely with L-type calcium channels like Cav1.2 which are responsible for the initial influx of calcium that activates RYR-2. This cooperation allows for the amplification of calcium signals needed for strong cardiac muscle contractions. Proper signaling in these pathways is necessary for maintenance of heart rhythm and contractile strength.
RYR-2 mutations associate with several cardiac disorders including catecholaminergic polymorphic ventricular tachycardia (CPVT) and arrhythmogenic right ventricular dysplasia type 2 (ARVD2). These conditions involve irregular heartbeats often triggered by physical exercise or stress. The relationship between RYR-2 and these disorders connects through its dysfunction in calcium signaling regulation which impacts heart rhythm stability. The malfunctioning of RYR-2 also affects related proteins like FKBP12.6 contributing further to disrupted calcium homeostasis and arrhythmic events.