Abcam, ab196409, Recombinant human EZH2 + EED + SUZ12 protein

Size: 50µg
Recombinant human EZH2 + EED + SUZ12 protein is a Human Full Length protein, in the 2 to 739 aa range, expressed in Baculovirus infected Sf9 cells, with >82%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>82% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: 0.08 pmole/min/μg.Assay conditions; 50 μl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 μM S-adenosylmethionine, and 250-2000 ng ab196409 is added to the wells coated with histone H3 substrate on a Neutravidin black plate (ThermoFisher). Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Add HRP chemiluminescent substrates and read luminescence.,
Accession:Q15022,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

Product details:
Complex of Human EZH2 (NM_004456), aa2-end with N-terminal His tag, MW= 86 kDa, Human EED (NM_003797), aa2-end with N-terminal DDDDK tag, MW= 51 kDa, and Human SUZ12 (NM_015355), aa2-end with N-terminal His tag, MW = 87 kDa, co-expressed in a baculovirus expression system.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The EZH2 + EED + SUZ12 complex also called the Polycomb Repressive Complex 2 (PRC2) plays a significant role in gene silencing through methylation of histone H3 at lysine 27 (H3K27me3). EZH2 acts as the catalytic subunit while EED and SUZ12 stabilize and enhance its catalytic activity. The mass of EZH2 is approximately 83 kDa. This complex is usually expressed in the nucleus where it performs its function in epigenetic regulation.
Biological function summary
PRC2 is essential for maintaining the silenced state of target genes. It acts as a part of the Polycomb-group (PcG) proteins which are important for transcriptional repression. Through their activity these complexes preserve the repression of developmental genes and prevent abnormal activation during cell differentiation and proliferation. PRC2 often teams up with other chromatin modifiers and DNA-binding proteins to accurately target specific genomic loci.
Pathways
EZH2 + EED + SUZ12 functions in the epigenetic regulatory pathway influencing major signaling pathways such as the Wnt and Notch pathways. Through its role in these pathways PRC2 regulates gene expression and is directly tied to critical cellular processes like cell cycle control and differentiation. Other proteins such as members of the SWI/SNF complex often counteract the function of PRC2 to balance gene expression patterns.
The abnormal regulation of EZH2 + EED + SUZ12 links to cancer and developmental disorders. Overexpression or mutation of EZH2 contributes to oncogenic processes in several types of cancer including prostate cancer. In the context of these diseases PRC2 interacts with additional oncogenic factors such as MYC promoting tumor progression. Furthermore disruptions in PRC2 function have connections to developmental disorders like Weaver syndrome through improper regulation of growth-regulating genes.